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KMID : 0894520130170030221
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Development & Reproduction
2013 Volume.17 No. 3 p.221 ~ p.229
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Expression Analysis of Cathepsin F during Embryogenesis and Early Developmental Stage in Olive Flounder (Paralichthys olivaceus)
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Lee Jang-Wook
Lee Young-Mee
Yang Hyun
Noh Jae-Koo
Kim Hyun-Chul
Park Choul-Ji
Park Jong-Won
Hwang In-Joon
Kim Sung-Yeon
Lee Jeong-Ho
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Abstract
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Cathepsins are members of the multigene family of lysosomal cysteine proteinases and have regulated function in several life processes. The potential role of cathepsin F cysteine gene was expected as protease in the yolk processing mechanism during early developmental stage, but expression analysis was unknown after fertilization. The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with 87-98% identity. In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age flounder. Tissue distribution of the cathepsin F mRNA has been shown to be ubiquitous and constitutive pattern regardless of age in each group, although derived from cDNA library using liver sample. The mRNA level of cathepsin F more increased as developmental proceed during embryogenesis and early developmental stage, especially increased in the blastula, hatching stage and 3 days post hatching (dph). As a result, it may suggest that the proteolysis of yolk proteins (YPs) has been implicated as a mechanism for nutrient supply during early larval stages in olive flounder.
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KEYWORD
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Olive flounder, Paralichthys olivaceus, Cysteine proteinase, Cathepsin F, Developmental stage, Gene expression, Yolk protein, Yolk syncytial layer (YSL)
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